Human Serum Albumin And Hydroxy Ethyl Starch Are Protective Alone And In Combination For Human Hematopoietic Stem Cells Stored at -80ºC With 5% Dimethylsulphoxide

Volume 7, Issue 1, February 2023     |     PP. 1-10      |     PDF (970 K)    |     Pub. Date: January 1, 2023
DOI: 10.54647/pm31186    100 Downloads     106304 Views  

Author(s)

Ivan Tonev, National Specialized Hospital for Active Treatment of Hematological Diseases, Sofia, Bulgaria
Milcho Mincheff, National Specialized Hospital for Active Treatment of Hematological Diseases, Sofia, Bulgaria

Abstract
The preservation of hematopoietic stem cells (HSC), which allows postponing of their transplantation is by cryoprotection. Dimethyl sulfoxide (DMSO) is a widely used intracellular cryoprotectant which is toxic for cells and patients at temperature above 0 ºC. A possible approach to reduce these toxicities is with addition of extracellular cryoprotectors as hydroxyethyl starch (HES) and plasma proteins to allow the use of DMSO in lower final concentration. We tested the protective role of HES and the plasma protein human serum albumin (HSA), a for human hematopoietic stem cells, subjected either to osmotic stress (a major factor in cellular injury during slow freezing) or to cryopreservation by unprogrammed freezing and storage at -80ºC. The viability was tested by trypan-blue exclusion assay.
HES has a protective effect on HSCs both against osmotic stress and cryopreservation and reduces cell aggregation. Addition of HSA to the cryoprotective solution improves viability and reduces cell clumping after thawing. Cryopreservation of HSCs with final 5% DMSO concentrations can be optimized by the addition of extracellular agents such as HES and HSA. This reduces DMSO toxicity to both HSCs and to patients during transplantation.

Keywords
DMSO concentration, extracellular cryoprotectors, -80 degrees, hydroxyethylstarch, HES, human albumin, HAS, hematopoietic stem cells, HSCs

Cite this paper
Ivan Tonev, Milcho Mincheff, Human Serum Albumin And Hydroxy Ethyl Starch Are Protective Alone And In Combination For Human Hematopoietic Stem Cells Stored at -80ºC With 5% Dimethylsulphoxide , SCIREA Journal of Medicine. Volume 7, Issue 1, February 2023 | PP. 1-10. 10.54647/pm31186

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